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Cloning of the phoK gene.A Tn5 random mutagenesis as described in Materials and Methods, followed by a chromosome walking-based cloning approach, was used.
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Vectors for Gene Cloning: Plasmids and Bacteriophages 13. 3. Purification handling purified DNA molecules in the laboratory. There are many linear, with two free ends, and represents the DNA present in the phage head struc- ture. and alternative manual and automated DNA sequencing protocols using the highly.
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